Observation of Fast Two-Dimensional NMR Spectra during Protein Folding Using Polarization Transfer from Hyperpolarized Water. Academic Article uri icon

abstract

  • Nuclear spin hyperpolarized water is utilized to obtain protein spectra not only in the folded state but also during the refolding process. Polarization transfer to Ribonuclease Sa through proton exchange and the nuclear Overhauser effect (NOE) results in NMR signal enhancements of amide protons by up to 24-fold. These enhancements enable the measurement of fast two-dimensional NMR spectra on the same time scale as the folding. Resolved amide proton signals corresponding to the folded protein are observed both under folded and refolding conditions, whereby the refolding protein shows smaller transferred signals. Residue-specific evaluation of contributions to the polarization transfer indicates that signals attributed to a relayed intramolecular NOE are not observable in the refolding experiment. These differences are explained by the absence of long-range contacts and faster molecular motions in the unfolded protein. Applications of this method include accessing residue-specific information on structure and dynamics during multistate protein folding.

published proceedings

  • J Phys Chem Lett

altmetric score

  • 2.35

author list (cited authors)

  • Kim, J., Mandal, R., & Hilty, C.

citation count

  • 14

complete list of authors

  • Kim, Jihyun||Mandal, Ratnamala||Hilty, Christian

publication date

  • January 2019