Carboxyl-coated magnetic nanoparticles for mRNA isolation and extraction of supercoiled plasmid DNA.

abstract

• Carboxyl-coated magnetic nanoparticles (MNPs) were used to demonstrate dual functionality: isolation of messenger RNA (mRNA) from mammalian cells and extraction of the supercoiled (sc) form of plasmid DNA (pDNA) from agarose gel. These MNPs were attached with 5'-NH(2)-tagged oligo-(dT)(25) primer and were used to isolate mRNA from breast cancer cells. The isolated mRNA was used for amplification of beta-actin to confirm the compatibility. These MNPs were also used to extract the sc form of pDNA from agarose gel. The compatibility of the pDNA was demonstrated by restriction digestion. Both of these methodologies are simple, inexpensive (compared with existing kits), and efficient.

authors

• Roy Sarkar, Tapasree

published proceedings

• Anal Biochem

altmetric score

• 3

author list (cited authors)

• Sarkar, T. R., & Irudayaraj, J.

citation count

• 42

complete list of authors

• Sarkar, Tapasree Roy||Irudayaraj, Joseph

publication date

• January 2008

publisher

• Elsevier  Publisher

published in

• Analytical Biochemistry  Journal

keywords

• Carboxylic Acids
• DNA, Superhelical
• Electrophoresis, Agar Gel
• Microscopy, Electron, Transmission
• Nanoparticles
• Nanotechnology
• Plasmids
• RNA, Messenger

Digital Object Identifier (DOI)

• 10.1016/j.ab.2008.04.016

start page

• 130

end page

• 132

volume

• 379

issue

• 1

URL

• http://dx.doi.org/10.1016/j.ab.2008.04.016