Biosynthesis of GDP-d-glycero--d-manno-heptose for the Capsular Polysaccharide of Campylobacter jejuni. Academic Article

abstract

• The capsular polysaccharide (CPS) structure of Campylobacter jejuni contributes to its robust fitness. Many strains contain heptose moieties in their CPS units. The precursor heptose is GDP-d-glycero--d-manno-heptose; modifications to the stereochemistry at C3-C6 as well as additions of methyl and phosphoramidate groups lend to the hypervariability of the C. jejuni CPS structures. Synthesis of GDP-d-glycero--d-manno-heptose has been described previously, but using enzymes from Aneurinibacillus thermoaerophilus DSM 10155. Here we describe the complete synthesis of GDP-d-glycero--d-manno-heptose using enzymes from C. jejuni NTCC 11168: Cj1152 and Cj1423-Cj1425. Our results yield kinetic parameters for these enzymes and outline a successful strategy for milligram-gram scale synthesis of GDP-d-glycero--d-manno-heptose. This achievement is critical for the characterization of other carbohydrate tailoring enzymes, which are expected to utilize GDP-d-glycero--d-manno-heptose for the biosynthesis of more complex carbohydrates in the CPS of C. jejuni.

authors

• Raushel, Frank

published proceedings

• Biochemistry

author list (cited authors)

• Huddleston, J. P., & Raushel, F. M.

citation count

• 13

complete list of authors

• Huddleston, Jamison P||Raushel, Frank M

publisher

• American Chemical Society (ACS)  Publisher

published in

• Biochemistry  Journal

keywords

• Bacterial Capsules
• Bacterial Proteins
• Campylobacter Jejuni
• Guanosine Diphosphate
• Heptoses
• Polysaccharides
• Protein Biosynthesis

Digital Object Identifier (DOI)

• 10.1021/acs.biochem.9b00548

start page

• 3893

end page

• 3902

volume

• 58

issue

• 37

URL

• http://dx.doi.org/10.1021/acs.biochem.9b00548