Tau phosphorylation regulates the interaction between BIN1's SH3 domain and Tau's proline-rich domain. Academic Article uri icon


  • INTRODUCTION: The application of high-throughput genomic approaches has revealed 24 novel risk loci for Alzheimer's disease (AD). We recently reported that the bridging integrator 1 (BIN1) risk gene is linked to Tau pathology. RESULTS: We used glutathione S-transferase pull-down assays and nuclear magnetic resonance (NMR) experiments to demonstrate that BIN1 and Tau proteins interact directly and then map the interaction between BIN1's SH3 domain and Tau's proline-rich domain (PRD) . Our NMR data showed that Tau phosphorylation at Thr231 weakens the SH3-PRD interaction. Using primary neurons, we found that BIN1-Tau complexes partly co-localize with the actin cytoskeleton; however, these complexes were not observed with Thr231-phosphorylated Tau species. CONCLUSION: Our results show that (i) BIN1 and Tau bind through an SH3-PRD interaction and (ii) the interaction is downregulated by phosphorylation of Tau Thr231 (and potentially other residues). Our study sheds new light on regulation of the BIN1/Tau interaction and opens up new avenues for exploring its complex's role in the pathogenesis of AD.

published proceedings

  • Acta Neuropathol Commun

altmetric score

  • 10

author list (cited authors)

  • Sottejeau, Y., Bretteville, A., Cantrelle, F., Malmanche, N., Demiaute, F., Mendes, T., ... Lambert, J.

citation count

  • 53

complete list of authors

  • Sottejeau, Yoann||Bretteville, Alexis||Cantrelle, Fran├žois-Xavier||Malmanche, Nicolas||Demiaute, Florie||Mendes, Tiago||Delay, Charlotte||Alves Dos Alves, Harmony||Flaig, Amandine||Davies, Peter||Dourlen, Pierre||Dermaut, Bart||Laporte, Jocelyn||Amouyel, Philippe||Lippens, Guy||Chapuis, Julien||Landrieu, Isabelle||Lambert, Jean-Charles

publication date

  • September 2015