Tau phosphorylation regulates the interaction between BIN1's SH3 domain and Tau's proline-rich domain.
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INTRODUCTION: The application of high-throughput genomic approaches has revealed 24 novel risk loci for Alzheimer's disease (AD). We recently reported that the bridging integrator 1 (BIN1) risk gene is linked to Tau pathology. RESULTS: We used glutathione S-transferase pull-down assays and nuclear magnetic resonance (NMR) experiments to demonstrate that BIN1 and Tau proteins interact directly and then map the interaction between BIN1's SH3 domain and Tau's proline-rich domain (PRD) . Our NMR data showed that Tau phosphorylation at Thr231 weakens the SH3-PRD interaction. Using primary neurons, we found that BIN1-Tau complexes partly co-localize with the actin cytoskeleton; however, these complexes were not observed with Thr231-phosphorylated Tau species. CONCLUSION: Our results show that (i) BIN1 and Tau bind through an SH3-PRD interaction and (ii) the interaction is downregulated by phosphorylation of Tau Thr231 (and potentially other residues). Our study sheds new light on regulation of the BIN1/Tau interaction and opens up new avenues for exploring its complex's role in the pathogenesis of AD.
author list (cited authors)
Sottejeau, Y., Bretteville, A., Cantrelle, F., Malmanche, N., Demiaute, F., Mendes, T., ... Lambert, J.
complete list of authors
Sottejeau, Yoann||Bretteville, Alexis||Cantrelle, François-Xavier||Malmanche, Nicolas||Demiaute, Florie||Mendes, Tiago||Delay, Charlotte||Alves Dos Alves, Harmony||Flaig, Amandine||Davies, Peter||Dourlen, Pierre||Dermaut, Bart||Laporte, Jocelyn||Amouyel, Philippe||Lippens, Guy||Chapuis, Julien||Landrieu, Isabelle||Lambert, Jean-Charles