Supplemental Antibiotics in a Commercial Extender for Stallion Semen.
Academic Article
Overview
Research
Identity
Additional Document Info
Other
View All
Overview
abstract
Commonly marketed semen extenders contain various antibiotic types and concentrations to control bacterial growth from stallion's external genitalia. An experiment was conducted to test the effects of supplemental amikacin disulfate (1,000g/mL)+ potassium penicillin G (1,000 IU/ML: INRA-AP), or ticarcillin-clavulanate (1,000g/mL: INRA-TIM) in the INRA 96 extender, on sperm function and antimicrobial activity, compared with extender without supplemental antibiotics (INRA-C). In freshly extended semen (Time 30m), no differences were observed among the three treatment groups for sperm motion characteristics or plasma membrane intactness (P > .05). Following cooled storage (Time 24h), sperm progressive motility and straightness were higher in INRA-AP, as compared to INRA-C or INRA-TIM (P < .05). For both time points, INRA-AP yielded lower bacterial colony-forming units (CFU/mL) than INRA-TIM or INRA-C (P < .05). In addition, INRA-AP yielded a higher proportion of culture plates with no growth (59%), than INRA-TIM (14%) or INRA-C (22%; P < .05). These findings suggest that INRA 96 extender can be supplemented with the tested concentrations of amikacin disulfate+ potassium penicillin G to improve its antimicrobial effectiveness without impairing sperm quality.
