Estrogen-induced signaling attenuates soluble A peptide-mediated dysfunction of pathways in synaptic plasticity.
- Additional Document Info
- View All
Neuromodulation of synaptic plasticity by 17-estradiol (E2) is thought to influence information processing and storage in the cortex and hippocampus. Because E2 rapidly affects cortical memory and synaptic plasticity, we examined its effects on phosphorylation of calcium/calmodulin-dependent protein kinase II (CaMKII), extracellular signal-regulated kinase (ERK), and alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) [AMPA-type glutamate receptor subunit 1 (GluR1 subunit)], all of which are important for the induction and maintenance of synaptic plasticity and memory. Acute E2 treatment resulted in an increased temporal and spatial phosphorylation pattern of CaMKII, ERK, and AMPAR (GluR1 subunit). By using inhibitors, we were able to attribute GluR1 phosphorylation to CaMKII at serine 831, and we also found that E2 treatment increased GluR1 insertion into the surface membrane. Because soluble amyloid-beta (A) oligomers inhibit CaMKII and ERK activation, which is necessary for synaptic plasticity, we also tested E2's ability to ameliorate A-induced dysfunction of synaptic plasticity. We found that estrogen treatment in neuronal culture, slice culture, and in vivo, ameliorated A oligomer-induced inhibition of CaMKII, ERK, and AMPAR phosphorylation, and also ameliorated the A oligomer-induced reduction of dendritic spine density in a CaMKII-dependent manner. These phosphorylation events are correlated with the early stage of inhibitory avoidance learning, and our data show that E2 improved inhibitory avoidance memory deficits in animals treated with soluble A oligomers. This study identifies E2-induced signaling that attenuates soluble A peptide-mediated dysfunction of pathways in synaptic plasticity.
author list (cited authors)
Logan, S. M., Sarkar, S. N., Zhang, Z., & Simpkins, J. W.
complete list of authors
Logan, Shaun M||Sarkar, Saumyendra N||Zhang, Zhang||Simpkins, James W