Defect driven gelation of 2D nano-assemblies and polymeric binder for bone tissue engineering
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2019 Omnipress - All rights reserved. Statement of purpose: Tissue engineering has emerged as a viable approach in restoring function to tissues and organs through deposition of therapeutic cells in 3D scaffold. A suitable scaffold should not only be mechanically robust, biodegradable and biocompatible but also permit cellular growth and differentiation. Recently there has been heightened interest in using two-dimensional (2D) nanomaterials to enhance surface and bulk properties of the polymeric scaffolds for bone tissue engineering [1]. Bioactivity of orthopaedic materials is characterized by its ability in stimulating bone growth through formation of apatite on its surface influencing cellular attachment and osteogenic differentiation. Transition metal dichalcogenides (TMDs) are a class of 2D nanomaterials possessing lattice atomic defects, high electron density and offer centers for binding thiolated molecules. In this work, the planar and atomic edge defects of MoS2 a 2D TMD have been utilised for establishing chemical conjugation with thiolated gelatin without a chemical crosslinker or UV exposure to synthesize hydrogels. These hydrogels possess enhanced mechanical properties, cellular biocompatibility and provide nanotopographies which would serve as site for apatite crystal nucleation. Thiolated gelatin due to its excellent biocompatibility, biodegradability and non-immunogenicity forms a superior platform for bone tissue engineering. Initially, the concentrations of MoS2 and thiolated gelatin will be optimised to obtain hydrogels with superior mechanical properties. Subsequently, cellular response will be evaluated by seeding hMSCs on these hydrogels.
