(Re)defining sensitivity of chemical imaging Conference Paper uri icon


  • 2019 SPIE. To successfully develop and manufacture a generic drug product, the latter is expected to be bioequivalent to its referencelisted drug, i.e. to show no significant difference in the rate and extent of absorption of the active pharmaceutical ingredient. Optical spectroscopy methods based on vibrational spectroscopy imaging have recently attracted significant attention as potentially viable approaches to quantify drugs' pharmacokinetics in vivo. However, substantial hurdles still exist due to signal interference from surrounding tissues, significant attenuation of signal in the depth of tissue to optical absorption and scattering, and a lack of quantifiable ways of assessing the signal generated from a drug compound in the depth of a tissue. In this report, we evaluated the major challenges of quantification and sensitive and reproducible analysis of drug distribution in tissues using Raman spectroscopy. Specific attention is given to the noise assessment, which affects both the sensitivity and reproducibility of data.

name of conference

  • Visualizing and Quantifying Drug Distribution in Tissue III

published proceedings


author list (cited authors)

  • O'Connor, S. P., Lalonde, J., Nodurft, D. T., & Yakovlev, V. V.

citation count

  • 0

complete list of authors

  • O'Connor, Sean P||Lalonde, Joshua||Nodurft, Dawson T||Yakovlev, Vladislav V

editor list (cited editors)

  • Evans, C. L., & Chan, K. F.

publication date

  • March 2019