Investigation of reaction mechanisms of cytochrome c and mitochondria with transient absorption spectroscopy Conference Paper uri icon

abstract

  • COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. We performed Transient Absorption Spectroscopy (TAS) on samples of cytochrome c in its reduced and oxidized states as well as mitochondria, and we use kinetic analysis to determine the decay rates of the transients. We also tested the samples following red light exposures to determine if there were any changes in transient decay rates. Mitochondria were isolated from hTERT-RPE1 cells and were tested in a glutamate buffer solution while cytochrome c was tested in a Sodium Phosphate solution (Na2PO4). Femtosecond TAS was performed with a pump pulse with wavelength centered at 418 nm and a supercontinuum probe pulse with a spectrum between 440 and 760 nm. Red light illuminations were performed with a 635 nm continuous wave light source at an intensity of 1.6 mW/cm2 for 0.5 hours. We find transients and kinetic decay rates for reduced and oxidized cytochrome c that are consistent with previous literature. However, we do not find any transients for mitochondria under our current TAS testing parameters, and we expect that this may be due to low Signalto- Noise ratio or choice of pump wavelength.

name of conference

  • Mechanisms of Photobiomodulation Therapy XIV

published proceedings

  • Mechanisms of Photobiomodulation Therapy XIV

author list (cited authors)

  • O'Connor, S. P., Lalonde, J. W., Rickman, J. M., Noojin, G. D., Pope, N. J., Denton, M. L., Scully, M. O., & Yakovlev, V. V.

editor list (cited editors)

  • Hamblin, M. R., Carroll, J. D., & Arany, P.

publication date

  • January 1, 2019 11:11 AM

publisher