Hot Spot-Localized Artificial Antibodies for Label-Free Plasmonic Biosensing. Academic Article uri icon


  • The development of biomolecular imprinting over the last decade has raised promising perspectives in replacing natural antibodies with artificial antibodies. A significant number of reports have been dedicated to imprinting of organic and inorganic nanostructures, but very few were performed on nanomaterials with a transduction function. Herein we describe a relatively fast and efficient plasmonic hot spot-localized surface imprinting of gold nanorods using reversible template immobilization and siloxane co-polymerization. The technique enables a fine control of the imprinting process at the nanometer scale and provides a nanobiosensor with high selectivity and reusability. Proof of concept is established by the detection of neutrophil gelatinase-associated lipocalin (NGAL), a biomarker for acute kidney injury, using localized surface plasmon resonance spectroscopy. The work represents a valuable step towards plasmonic nanobiosensors with synthetic antibodies for label-free and cost-efficient diagnostic assays. We expect that this novel class of surface imprinted plasmonic nanomaterials will open up new possibilities in advancing biomedical applications of plasmonic nanostructures.

published proceedings

  • Adv Funct Mater

altmetric score

  • 22.5

author list (cited authors)

  • Abbas, A., Tian, L., Morrissey, J. J., Kharasch, E. D., & Singamaneni, S.

citation count

  • 83

complete list of authors

  • Abbas, Abdennour||Tian, Limei||Morrissey, Jeremiah J||Kharasch, Evan D||Singamaneni, Srikanth

publication date

  • April 2013