Biological variation of serum canine calprotectin concentrations as measured by ELISA in healthy dogs. Academic Article uri icon


  • Calprotectin is a useful biomarker of inflammation in dogs. However, the biological variation of serum canine calprotectin is unknown. Indices of biological variation were determined in serial serum samples (n=147) from 11 healthy dogs (males/females: 4/7, median age: 5 years): analytical (3.0%), intra-individual (29.9%), and inter-individual variation (33.2%), reciprocal index of individuality (1.1), and index of heterogeneity (4.9). Serum calprotectin concentrations measured by ELISA and by the previous radioimmunoassay were highly correlated, but a constant and proportional bias exists between both assays. A de novo ELISA-reference interval (RI) for serum calprotectin concentration was established (0.6-11.8mg/L). Moderate changes in serum calprotectin (minimum critical difference: 6.4mg/L) between sequential measurements are needed to be considered relevant, and a population-based RI may or may not be appropriate for serum calprotectin.

published proceedings

  • Vet J

altmetric score

  • 0.5

author list (cited authors)

  • Heilmann, R. M., Ruaux, C. G., Grtzner, N., Cranford, S. M., Bridges, C. S., & Steiner, J. M.

citation count

  • 3

complete list of authors

  • Heilmann, RM||Ruaux, CG||Gr├╝tzner, N||Cranford, SM||Bridges, CS||Steiner, JM

publication date

  • May 2019