The present study features a two-pass rectangular channel with an aspect ratio (AR) = 4:1 in the first pass and an AR = 2:1 in the second pass after a 180-deg tip turn. In addition to the smooth-wall case, ribs with a profiled cross section are placed at 60 deg to the flow direction on both the leading and trailing surfaces in both passages (P/e = 10, e/Dh ∼ 0.11, parallel and in-line). Regionally averaged heat transfer measurement method was used to obtain the heat transfer coefficients on all internal surfaces. The Reynolds number (Re) ranges from 10,000 to 70,000 in the first passage, and the rotational speed ranges from 0 to 400 rpm. Under pressurized condition (570 kPa), the highest rotation number achieved was Ro = 0.39 in the first passage and 0.16 in the second passage. The results showed that the turn-induced secondary flows are reduced in an accelerating flow. The effects of rotation on heat transfer are generally weakened in the ribbed case than the smooth case. Significant heat transfer reduction (∼30%) on the tip wall was seen in both the smooth and ribbed cases under rotating condition. Overall pressure penalty was reduced for the ribbed case under rotation. Reynolds number effect was found noticeable in the current study. The heat transfer and pressure drop characteristics are sensitive to the geometrical design of the channel and should be taken into account in the design process.