Sortase A-mediated multi-functionalization of protein nanoparticles
- Additional Document Info
- View All
We report here a new strategy to enable fast, covalent, and site-directed functionalization of protein nanoparticles using Sortase A-mediated ligation using functional proteins ranging from monomeric to large tetrameric structures. Easy purification of the modified E2 nanoparticles is achieved by functionalization with a thermo-responsive elastin-like-peptide. The resulting protein nanoparticles remained intact and active even after repeated phase transitions, suggesting their use in biocatalysis, biosensing, and imaging applications.
author list (cited authors)
Chen, Q. i., Sun, Q., Molino, N. M., Wang, S., Boder, E. T., & Chen, W.