Sortase A-mediated multi-functionalization of protein nanoparticles Academic Article uri icon


  • We report here a new strategy to enable fast, covalent, and site-directed functionalization of protein nanoparticles using Sortase A-mediated ligation using functional proteins ranging from monomeric to large tetrameric structures. Easy purification of the modified E2 nanoparticles is achieved by functionalization with a thermo-responsive elastin-like-peptide. The resulting protein nanoparticles remained intact and active even after repeated phase transitions, suggesting their use in biocatalysis, biosensing, and imaging applications.

author list (cited authors)

  • Chen, Q. i., Sun, Q., Molino, N. M., Wang, S., Boder, E. T., & Chen, W.

citation count

  • 33

publication date

  • August 2015