Creation of artificial cellulosomes on DNA scaffolds by zinc finger protein-guided assembly for efficient cellulose hydrolysis.
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abstract
For the first time, artificial cellulosome structures were created on DNA scaffolds based on zinc finger protein (ZFP)-guided assembly. These resulting two-component cellulosome structures exhibited enhancement in cellulose hydrolysis compared to the non-complexed mixture depending on the number of CBMs and cellulases assembled.