Axial plane optical microscopy. Academic Article uri icon


  • We present axial plane optical microscopy (APOM) that can, in contrast to conventional microscopy, directly image a sample's cross-section parallel to the optical axis of an objective lens without scanning. APOM combined with conventional microscopy simultaneously provides two orthogonal images of a 3D sample. More importantly, APOM uses only a single lens near the sample to achieve selective-plane illumination microscopy, as we demonstrated by three-dimensional (3D) imaging of fluorescent pollens and brain slices. This technique allows fast, high-contrast, and convenient 3D imaging of structures that are hundreds of microns beneath the surfaces of large biological tissues.

published proceedings

  • Sci Rep

altmetric score

  • 3

author list (cited authors)

  • Li, T., Ota, S., Kim, J., Wong, Z. J., Wang, Y., Yin, X., & Zhang, X.

citation count

  • 39

complete list of authors

  • Li, Tongcang||Ota, Sadao||Kim, Jeongmin||Wong, Zi Jing||Wang, Yuan||Yin, Xiaobo||Zhang, Xiang

publication date

  • January 2014