Artificial scaffolds for enhanced biocatalysis. Academic Article

abstract

• Proteins are not designed to be standalone entities and must coordinate their collective action for optimum performance. Nature has developed through evolution the ability to colocalize the functional partners of a cascade enzymatic reaction in order to ensure efficient exchange of intermediates. Inspired by these natural designs, synthetic scaffolds have been created to enhance the overall biological pathway performance. In this chapter, we describe several DNA- and protein-based scaffold approaches to assemble artificial enzyme cascades for a wide range of applications. We highlight the key benefits and drawbacks of these approaches to provide insights on how to choose the appropriate scaffold for different cascade systems.

authors

• Sun, Qing

published proceedings

• Methods Enzymol

author list (cited authors)

• Sun, Q., Tsai, S., & Chen, W.

citation count

• 6

complete list of authors

• Sun, Qing||Tsai, Shen-Long||Chen, Wilfred

publication date

• January 2019

publisher

• Elsevier  Publisher

published in

• METHODS IN ENZYMOLOGY  Journal

keywords

• Bacteria
• Biocatalysis
• Biocompatible Materials
• Cascade
• Cellulosomes
• DNA
• Enzymes, Immobilized
• Metabolon
• Models, Molecular
• Scaffold
• Substrate Channeling

PubMed Central ID

• 30784409

Digital Object Identifier (DOI)

• 10.1016/bs.mie.2018.12.007

start page

• 363

end page

• 383

volume

• 617

URL

• http://dx.doi.org/10.1016/bs.mie.2018.12.007