Interruption of Somatic Embryogenesis in Daucus carota L. by 5-Bromodeoxyuridine. Academic Article

abstract

• Embryogenic Daucus carota L. cells grown in 9 micromolar 2.4-dichlorophenoxyacetic acid are resistant to greater than 5 micromolar 5-bromodeoxyuridine (BrdU). In contrast, 5 micromolar BrdU strongly inhibits somatic embryogenesis within 24 hours after transfer of cells to an auxin-free medium. DNA synthesis rates in control and BrdU-treated cultures are rapid and similar; however, the DNA content does not reach levels as great in the presence of BrdU as in control cultures. BrdU substitutes for thymidine in the DNA in 28% of the available sites 48 hours after auxin removal. Following DNA repair, somatic embryogenesis resumes. BrdU DNA incorporation leads to somatic embryogenesis inhibition and provides an alternative to auxin treatment for the interruption of carrot cell culture differentiation.

authors

• Nessler, Craig

published proceedings

• Plant Physiol

author list (cited authors)

• Thomas, J. C., Nessler, C., & Katterman, F.

citation count

• 7

complete list of authors

• Thomas, JC||Nessler, C||Katterman, F

publication date

• July 1989

publisher

• Oxford University Press (OUP)  Publisher

published in

• PLANT PHYSIOLOGY  Journal

keywords

• 0604 Genetics
• Basic Science
• Genetics

PubMed Central ID

• 16666898

Digital Object Identifier (DOI)

• 10.1104/pp.90.3.921

start page

• 921

end page

• 927

volume

• 90

issue

• 3

URL

• http://dx.doi.org/10.1104/pp.90.3.921