Laticifers in seedlings of Glaucium flavum Crantz were examined by electron microscopy. Laticifer initials first apeared in procambium of the radicle adjacent to the phloem about 48–72 h after germination. Differentiation of laticifer initials was characterized by the proliferation of numerous vesicles from dilation of endoplasmic reticulum. The usual complement of organelles was present in laticifer elements including a nucleus, mitochondria, dictyosomes, and plastids. Plastids were devoid of organized lamellae and starch grains but contained electron-dense, membrane-bound inclusions. Large crystalline bodies of unknown composition were also seen in the cytoplasm of some laticifer elements. Perforations were not observed in the longitudinal walls shared by adjacent laticifer elements; thus the laticifer system in this species can be classified as nonanastomosing. Transverse walls between laticifer elements remained intact until late in cell differentiation; however, large perforations did form in these walls as a result of the gradual removal of wall material at the perforation site.