Cellular localization of tyrosine decarboxylase expression in transgenic opium poppy and tobacco Academic Article

abstract

• Opium poppy, Papaver somniferum, is cultivated for its alkaloid-rich latex. Tyrosine decarboxylase (TyDC) is the first enzyme in poppy alkaloid biosynthesis and is encoded by a small gene family. A 2,060-bp promoter fragment of TyDC5 was translationally fused to the -glucuronidase (GUS) reporter gene and introduced into poppy and tobacco (Nicotiana tabacum). Transgenic seedlings were stained for GUS activity which localized to the xylem parenchyma in the shoots of poppy and tobacco. Roots of both species had similar expression patterns with staining in the vascular cylinder surrounding the xylem. No staining was observed in poppy laticifers suggesting that other TyDC genes may be expressed in latex or that alkaloid precursors are supplied to laticifers by adjacent cells.

authors

• Nessler, Craig

published proceedings

• PLANT CELL REPORTS

author list (cited authors)

• El-Ahmady, S. H., & Nessler, C. L.

citation count

• 7

complete list of authors

• El-Ahmady, SH||Nessler, CL

publication date

• January 2001

publisher

• Springer Nature  Publisher

published in

• Plant Cell Reports  Journal

keywords

• Alkaloid
• Laticifer
• Papaver Somniferum
• Tyrosine Decarboxylase Expression

Digital Object Identifier (DOI)

• 10.1007/s002990100331

start page

• 313

end page

• 317

volume

• 20

issue

• 4

URL

• http://dx.doi.org/10.1007/s002990100331

user-defined tag

• 3 Good Health and Well-Being