CYTOCHEMICAL-LOCALIZATION OF PECTINASE ACTIVITY IN LATICIFERS OF NERIUM-OLEANDER L

abstract

• A method is described for the cytochemical localization of pectinase activity at the ultrastructural level. The procedure involves the use of Benedict's reagent to form an electron-dense copper precipitate when reacted with reducing sugars liberated from exogenously supplied pectin. Using this technique, pectinase activity was examined in the nonarticulated, branched laticifers of Nerium oleander. Electron opaque crystalline deposits indicating the presence of pectolytic enzymes were identified in laticifer central vacuoles. Smaller amounts of reaction product were distributed along the middle lamella between laticifers and adjacent cells. This report represents the first direct evidence for the involvement of pectinase in intrusive growth of nonarticulated laticifers. 1984 Springer-Verlag.

authors

• Nessler, Craig

published proceedings

• PROTOPLASMA

author list (cited authors)

• ALLEN, R. D., & NESSLER, C. L.

citation count

• 21

complete list of authors

• ALLEN, RD||NESSLER, CL

publication date

• February 1984

publisher

• Springer Nature  Publisher

published in

• Protoplasma: an international journal of cell biology  Journal

keywords

• 31 Biological Sciences
• 3108 Plant Biology

Digital Object Identifier (DOI)

• 10.1007/BF01287819

start page

• 74

end page

• 78

volume

• 119

issue

• 1-2

URL

• http://dx.doi.org/10.1007/bf01287819