RAPID ANALYSIS OF OPIUM-ALKALOIDS BY ION-PAIR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY Academic Article

abstract

• A new procedure for the rapid analysis of four major opium alkaloids is described using ion-pair, reversedphase high performance liquid chromatography and recently developed radial compression column technology. The solvents were aqueous 10mM potassium perchlorate with 5.0mM n-butylamine (pH 3.0) and pure acetonitrile. A gradient was run for five minutes from 10% to 70% acetonitrile and held at that level for two minutes. Flow rates were 3 ml/min. Two column packing materials were compared with this solvent system: standard C-18 packing and a fully-end-capped C-18 material. Results obtained with the fully-end-capped C-18 packing and the two solvent ion-pair gradient showed elution times under six minutes, with greater sensitivity (<50 nanograms) than previous systems. 1983 Friedr. Vieweg & Sohn Verlagsgesellschaft mbH.

authors

• Nessler, Craig

published proceedings

• CHROMATOGRAPHIA

author list (cited authors)

• GALEWSKY, S., & NESSLER, C. L.

citation count

• 14

complete list of authors

• GALEWSKY, S||NESSLER, CL

publication date

• February 1984

publisher

• Springer Nature  Publisher

published in

• Chromatographia  Journal

keywords

• 34 Chemical Sciences
• 3401 Analytical Chemistry

Digital Object Identifier (DOI)

• 10.1007/BF02268463

start page

• 87

end page

• 90

volume

• 18

issue

• 2

URL

• http://dx.doi.org/10.1007/bf02268463