SOMATIC EMBRYOGENESIS IN THE OPIUM POPPY, PAPAVER-SOMNIFERUM Academic Article uri icon

abstract

  • A procedure is described for the induction of somatic embryos in the opium poppy. Papaver somniferum L. Callus was obtained from seedling hypocotyls on an agar solidified medium [Murashige and Skoog (1962) Physiol. Plant. 15: 473497] containing 0.25 mg/l (1.2 M) kinetin and 2.0 mg/l (10.7 M) naphtalene acetic acid (NAA). Suspension cultures were initiated from callus using a liquid medium in which 2.0 mg/l (9.0 M) 2,4dichlorophenoxyacetic acid was substituted for NAA. Meristemoids, spheres of closely packed cells, developed in suspensions and on the surface of a few callus cultures. Differentiation of meristemoids into somatic embryos was accomplished by removing growth regulators from the liquid medium. Embryoids appeared morphologically normal and similar to torpedo stage embryos, however, they possessed mature tracheary elements and laticifers in areas that should have contained only procambium. Whole plants have been obtained by placing embryos in the light on solid medium that also lacked growth regulators. Copyright 1982, Wiley Blackwell. All rights reserved

published proceedings

  • PHYSIOLOGIA PLANTARUM

author list (cited authors)

  • NESSLER, C. L.

citation count

  • 42

complete list of authors

  • NESSLER, CL

publication date

  • January 1982

publisher