Cloning and expression of a plant homologue of the small subunit of the Golgi-associated clathrin assembly protein AP19 from Camptotheca acuminata. Academic Article

abstract

• Clathrin-coated vesicles (CCVs) are involved in selective protein transport in eukaryotes. AP-1 and AP-2 are protein complexes found in the CCVs of the Golgi apparatus and the plasma membrane respectively. AP19 is the smallest polypeptide chain components of AP-1. We have identified a cDNA clone (CAP19) encoding a putative homologue for the assembly protein AP19 from the Chinese medicinal tree, Camptotheca acuminata. The deduced polypeptide contains 161 amino acids and has a predicted Mr of 18820. DNA blot analysis suggests that the AP19S of C. acuminata are encoded by a small gene family. CAP19 was expressed ubiquitously throughout the plant suggesting that it may be involved in general Golgi-mediated secretion.

authors

• Nessler, Craig

published proceedings

• Plant Mol Biol

altmetric score

• 3

author list (cited authors)

• Maldonado-Mendoza, I. E., & Nessler, C. L.

citation count

• 16

complete list of authors

• Maldonado-Mendoza, IE||Nessler, CL

publication date

• December 1996

publisher

• Springer Nature  Publisher

keywords

• Adaptor Protein Complex 1
• Adaptor Protein Complex Sigma Subunits
• Adaptor Proteins, Vesicular Transport
• Amino Acid Sequence
• Clathrin
• Cloning, Molecular
• DNA, Complementary
• Gene Expression
• Genes, Plant
• Golgi Apparatus
• Molecular Sequence Data
• Molecular Weight
• Nerve Tissue Proteins
• Phosphoproteins
• Plant Proteins
• Plants, Medicinal
• Sequence Alignment
• Vesicular Transport Proteins

PubMed Central ID

• 9002613

Digital Object Identifier (DOI)

• 10.1007/BF00041398

start page

• 1149

end page

• 1153

volume

• 32

issue

• 6

URL

• http://dx.doi.org/10.1007/bf00041398