Effects of BPA on expression of apoptotic genes and migration of ovine trophectoderm (oTr1) cells during the peri-implantation period of pregnancy
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Bisphenol A (BPA) is an endocrine-disrupting chemical used in the manufacture of many products used daily. In the present study, the effects of BPA (1 × 10-4 to 1 × 10-9 M) on migration and on the expression of some apoptotic genes were examined in vitro using ovine trophectoderm (oTr1) primary cell line. The results revealed that BPA at 1 × 10-9, 1 × 10-8 and 1 × 10-7M increased migration of oTr1 cells, while 1 × 10-6, 1 × 10-5 and 1 × 10-4 M BPA decreased cell migration. Regarding apoptosis, expression of the anti-apoptotic gene Bcl-2 mRNA was greater at 1 × 10-8 and 1 × 10-9 M BPA and was down-regulated at 1 × 10-4 to 1 × 10-7 M BPA; however, expression of pro-apoptotic genes (Bax, cathepsin B, caspase-3 and c-myc) was reduced at the higher concentrations of BPA. Results of this study suggest that BPA may impair implantation by decreasing migration of oTr1 cells and inhibiting apoptosis.
author list (cited authors)
Elmetwally, M. A., Halawa, A. A., Lenis, Y. Y., Tang, W., Wu, G., & Bazer, F. W.