Effects of BPA on expression of apoptotic genes and migration of ovine trophectoderm (oTr1) cells during the peri-implantation period of pregnancy. Academic Article uri icon

abstract

  • Bisphenol A (BPA) is an endocrine-disrupting chemical used in the manufacture of many products used daily. In the present study, the effects of BPA (110-4 to 110-9 M) on migration and on the expression of some apoptotic genes were examined in vitro using ovine trophectoderm (oTr1) primary cell line. The results revealed that BPA at 110-9, 110-8 and 110-7M increased migration of oTr1 cells, while 110-6, 110-5 and 110-4 M BPA decreased cell migration. Regarding apoptosis, expression of the anti-apoptotic gene Bcl-2 mRNA was greater at 110-8 and 110-9 M BPA and was down-regulated at 110-4 to 110-7 M BPA; however, expression of pro-apoptotic genes (Bax, cathepsin B, caspase-3 and c-myc) was reduced at the higher concentrations of BPA. Results of this study suggest that BPA may impair implantation by decreasing migration of oTr1 cells and inhibiting apoptosis.

published proceedings

  • Reprod Toxicol

author list (cited authors)

  • Elmetwally, M. A., Halawa, A. A., Lenis, Y. Y., Tang, W., Wu, G., & Bazer, F. W.

citation count

  • 7

complete list of authors

  • Elmetwally, Mohammed A||Halawa, Amal A||Lenis, Yasser Y||Tang, Wanjin||Wu, Guoyao||Bazer, Fuller W

publication date

  • January 2019