Genome Size Estimation and Quantitative Cytogenetics in Insects Chapter

abstract

• With care, it is possible using flow cytometry to create a precise and accurate estimate of the genome size of an insect that is useful for genomics, genetics, molecular/cell biology, or systematics. Genome size estimation is a useful first step in a complete genome sequencing project. The number of sequencing reads required to produce a given level of coverage depends directly upon the 1C amount of DNA per cell, while an even more critical need is an accurate 1C genome size estimate to compare against the final assembly. Here we present a detailed protocol to estimate genome size using flow cytometry. Published genome size estimates should be submitted to genomesize.comso that they are available to all.

authors

• Johnston, J

author list (cited authors)

• Johnston, J. S., Bernardini, A., & Hjelmen, C. E.

citation count

• 30

complete list of authors

• Johnston, JS||Bernardini, A||Hjelmen, CE

editor list (cited editors)

• Brown, S. J., & Pfrender, M. E.

Book Title

• Insect Genomics: Methods and Protocols

publication date

• January 2019

publisher

• Springer New York  Publisher

keywords

• Animals
• Chromatin Structure
• Chromosomal Sex Determination
• Cryptic Species
• Cytogenetic Analysis
• Endopolyploidy
• Flow Cytometry
• Genome Size
• Genome, Insect
• INSECTA
• Ploidy
• Sexual Dimorphism
• Underreplication

PubMed Central ID

• 30414107

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-8775-7_2

International Standard Book Number (ISBN) 13

• 978-1-4939-8775-7

start page

• 15

end page

• 26

URL

• http://dx.doi.org/10.1007/978-1-4939-8775-7_2