Comparing Arabidopsis receptor kinase and receptor protein-mediated immune signaling reveals BIK1-dependent differences. Academic Article uri icon

abstract

  • Pattern recognition receptors (PRRs) sense microbial patterns and activate innate immunity against attempted microbial invasions. The leucine-rich repeat receptor kinases (LRR-RK) FLS2 and EFR, and the LRR receptor protein (LRR-RP) receptors RLP23 and RLP42, respectively, represent prototypical members of these two prominent and closely related PRR families. We conducted a survey of Arabidopsis thaliana immune signaling mediated by these receptors to address the question of commonalities and differences between LRR-RK and LRR-RP signaling. Quantitative differences in timing and amplitude were observed for several early immune responses, with RP-mediated responses typically being slower and more prolonged than those mediated by RKs. Activation of RLP23, but not FLS2, induced the production of camalexin. Transcriptomic analysis revealed that RLP23-regulated genes represent only a fraction of those genes differentially expressed upon FLS2 activation. Several positive and negative regulators of FLS2-signaling play similar roles in RLP23 signaling. Intriguingly, the cytoplasmic receptor kinase BIK1, a positive regulator of RK signaling, acts as a negative regulator of RP-type immune receptors in a manner dependent on BIK1 kinase activity. Our study unveiled unexpected differences in two closely related receptor systems and reports a new negative role of BIK1 in plant immunity.

published proceedings

  • New Phytol

altmetric score

  • 4.75

author list (cited authors)

  • Wan, W., Zhang, L., Pruitt, R., Zaidem, M., Brugman, R., Ma, X., ... Nrnberger, T.

citation count

  • 60

complete list of authors

  • Wan, Wei-Lin||Zhang, Lisha||Pruitt, Rory||Zaidem, Maricris||Brugman, Rik||Ma, Xiyu||Krol, Elzbieta||Perraki, Artemis||Kilian, Joachim||Grossmann, Guido||Stahl, Mark||Shan, Libo||Zipfel, Cyril||van Kan, Jan AL||Hedrich, Rainer||Weigel, Detlef||Gust, Andrea A||Nürnberger, Thorsten

publisher