Isolation of a delta 6-desaturase gene from the cyanobacterium Synechocystis sp. strain PCC 6803 by gain-of-function expression in Anabaena sp. strain PCC 7120. Academic Article uri icon

abstract

  • The enzyme delta 6-desaturase is responsible for the conversion of linoleic acid (18:2) to gamma-linolenic acid (18:3 gamma). A cyanobacterial gene encoding delta 6-desaturase was cloned by expression of a Synechocystis genomic cosmid library in Anabaena, a cyanobacterium lacking delta 6-desaturase. Expression of the Synechocystis delta 6-desaturase gene in Anabaena resulted in the accumulation of gamma-linolenic acid (GLA) and octadecatetraenoic acid (18:4). The predicted 359 amino acid sequence of the Synechocystis delta 6-desaturase shares limited, but significant, sequence similarity with two other reported desaturases. Analysis of three overlapping cosmids revealed a delta 12-desaturase gene linked to the delta 6-desaturase gene. Expression of Synechocystis delta 6- and delta 12-desaturases in Synechococcus, a cyanobacterium deficient in both desaturases, resulted in the production of linoleic acid and gamma-linolenic acid.

published proceedings

  • Plant Mol Biol

altmetric score

  • 6

author list (cited authors)

  • Reddy, A. S., Nuccio, M. L., Gross, L. M., & Thomas, T. L.

citation count

  • 113

complete list of authors

  • Reddy, AS||Nuccio, ML||Gross, LM||Thomas, TL

publication date

  • May 1993