The carrot secreted glycoprotein gene EP1 is expressed in the epidermis and has sequence homology to Brassica S-locus glycoproteins.

abstract

• Non-embryogenic carrot suspension cells secrete the EP1 glycoprotein. A cDNA clone encoding EP1 was isolated and sequenced. The EP1 sequence revealed a region of homology with Brassica S-locus glycoprotein genes, an Arabidopsis S-like gene and putative S-like receptor protein kinases from maize and Arabidopsis. EP1 gene expression, analysed by in situ mRNA localization, was detected in cells located at the surface of the seedling: in the epidermis of the root, the hypocotyl and the cotyledons, in the root cap, and in a crescent of cells in the apical dome of the shoot. In developing seeds, expression was most pronounced in both the inner and outer integument epidermis.

authors

• Thomas, Terry

published proceedings

• Plant J

author list (cited authors)

• van Engelen, F. A., Hartog, M. V., Thomas, T. L., Taylor, B., Sturm, A., van Kammen, A., & de Vries, S. C.

citation count

• 28

complete list of authors

• van Engelen, FA||Hartog, MV||Thomas, TL||Taylor, B||Sturm, A||van Kammen, A||de Vries, SC

publication date

• November 1993

publisher

• Wiley  Publisher

published in

• The Plant Journal  Journal

keywords

• Amino Acid Sequence
• Base Sequence
• DNA, Complementary
• Genes, Plant
• Glycoproteins
• In Situ Hybridization
• Molecular Sequence Data
• Plant Proteins
• RNA, Messenger
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid
• Tissue Distribution
• Vegetables

PubMed Central ID

• 8275102

Digital Object Identifier (DOI)

• 10.1046/j.1365-313x.1993.04050855.x

start page

• 855

end page

• 862

volume

• 4

issue

• 5

URL

• http://dx.doi.org/10.1046/j.1365-313x.1993.04050855.x