The carrot secreted glycoprotein gene EP1 is expressed in the epidermis and has sequence homology to Brassica S-locus glycoproteins.
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Non-embryogenic carrot suspension cells secrete the EP1 glycoprotein. A cDNA clone encoding EP1 was isolated and sequenced. The EP1 sequence revealed a region of homology with Brassica S-locus glycoprotein genes, an Arabidopsis S-like gene and putative S-like receptor protein kinases from maize and Arabidopsis. EP1 gene expression, analysed by in situ mRNA localization, was detected in cells located at the surface of the seedling: in the epidermis of the root, the hypocotyl and the cotyledons, in the root cap, and in a crescent of cells in the apical dome of the shoot. In developing seeds, expression was most pronounced in both the inner and outer integument epidermis.
author list (cited authors)
van Engelen, F. A., Hartog, M. V., Thomas, T. L., Taylor, B., Sturm, A., van Kammen, A., & de Vries, S. C.
complete list of authors
van Engelen, FA||Hartog, MV||Thomas, TL||Taylor, B||Sturm, A||van Kammen, A||de Vries, SC