Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel
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ORAI1 constitutes the pore-forming subunit of the calcium release-activated calcium (CRAC) channel, a prototypical store-operated channel that is essential for the activation of cells of the immune system. Here we describe a convenient yet powerful cross-linking approach to examine the pore architecture of CRAC channels using ORAI1 proteins engineered to contain one or two cysteine residues. The generalizable cross-linking in situ approach can also be readily extended to study other integral membrane proteins expressed in various types of cells.
author list (cited authors)
Ma, G., He, L., Jing, J. i., Tan, P., Huang, Y., & Zhou, Y.