Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel. Chapter uri icon

abstract

  • ORAI1 constitutes the pore-forming subunit of the calcium release-activated calcium (CRAC) channel, a prototypical store-operated channel that is essential for the activation of cells of the immune system. Here we describe a convenient yet powerful cross-linking approach to examine the pore architecture of CRAC channels using ORAI1 proteins engineered to contain one or two cysteine residues. The generalizable cross-linking in situ approach can also be readily extended to study other integral membrane proteins expressed in various types of cells.

altmetric score

  • 1.5

author list (cited authors)

  • Ma, G., He, L., Jing, J. i., Tan, P., Huang, Y., & Zhou, Y.

citation count

  • 1

complete list of authors

  • Ma, Guolin||He, Lian||Jing, Ji||Tan, Peng||Huang, Yun||Zhou, Yubin

Book Title

  • CRAC CHANNEL: METHODS AND PROTOCOLS

publication date

  • January 2018