Engineered Cross-Linking to Study the Pore Architecture of the CRAC Channel Chapter uri icon


  • ORAI1 constitutes the pore-forming subunit of the calcium release-activated calcium (CRAC) channel, a prototypical store-operated channel that is essential for the activation of cells of the immune system. Here we describe a convenient yet powerful cross-linking approach to examine the pore architecture of CRAC channels using ORAI1 proteins engineered to contain one or two cysteine residues. The generalizable cross-linking in situ approach can also be readily extended to study other integral membrane proteins expressed in various types of cells.

altmetric score

  • 1.25

author list (cited authors)

  • Ma, G., He, L., Jing, J. i., Tan, P., Huang, Y., & Zhou, Y.

citation count

  • 0

Book Title

  • The CRAC Channel

publication date

  • September 2018