Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells. Chapter

abstract

• Induced by the depletion of ER calcium store, the calcium influx through calcium release-activated calcium (CRAC) channels is an ubiquitous way of Ca2+ influx for most cell types. This process is mediated by STIM protein, ER calcium sensor, and PM localized Orai calcium channels. Biophysical characterization of this STIM-Orai-mediated current, or ICRAC, with whole-cell patch-clamp technique is essential for revealing the molecular mechanisms underlying the process of STIM-Orai activation or modulation. Here we describe one commonly used procedure of monitoring CRAC activity with whole-cell patch-clamp technique.

authors

• Zhou, Yubin

author list (cited authors)

• Zhang, Y., Zheng, S., Zhou, Y., Gill, D. L., & Wang, Y.

citation count

• 0

complete list of authors

• Zhang, Yiming||Zheng, Sisi||Zhou, Yubin||Gill, Donald L||Wang, Youjun

Book Title

• The CRAC Channel

publication date

• September 2018

publisher

• Springer New York  Publisher

published in

• Methods Mol Biol  Journal

keywords

• Calcium Release Activated Calcium Channels
• Calcium Release-activated Calcium (crac) Channel
• Cell Line
• Data Analysis
• Endoplasmic Reticulum
• Gene Expression
• Humans
• Icrac
• Ion Channel Gating
• Patch-Clamp Techniques
• Software
• Store-operated Ca2+ Entry (soce)
• Stromal Interaction Molecules
• Transfection
• Whole-cell Patch Clamp

PubMed Central ID

• 30203273

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-8704-7_1

International Standard Book Number (ISBN) 13

• 9781493987023

start page

• 1

end page

• 16

volume

• 1843