Patch-Clamp Recording of the CRAC Channel Current in STIM-Orai Overexpressing Cells.
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Induced by the depletion of ER calcium store, the calcium influx through calcium release-activated calcium (CRAC) channels is an ubiquitous way of Ca2+ influx for most cell types. This process is mediated by STIM protein, ER calcium sensor, and PM localized Orai calcium channels. Biophysical characterization of this STIM-Orai-mediated current, or ICRAC, with whole-cell patch-clamp technique is essential for revealing the molecular mechanisms underlying the process of STIM-Orai activation or modulation. Here we describe one commonly used procedure of monitoring CRAC activity with whole-cell patch-clamp technique.
author list (cited authors)
Zhang, Y., Zheng, S., Zhou, Y., Gill, D. L., & Wang, Y.
complete list of authors
Zhang, Yiming||Zheng, Sisi||Zhou, Yubin||Gill, Donald L||Wang, Youjun