Deuterium isotope effects and fractionation factors of hydrogen-bonded A:T base pairs of DNA. Academic Article

abstract

• Deuterium isotope effects and fractionation factors of N1.H3-N3 hydrogen bonded Watson-Crick A:T base pairs of two DNA dodecamers are presented here. Specifically, two-bond deuterium isotope effects on the chemical shifts of (13)C2 and (13)C4, (2)delta(13)C2 and (2)delta(13)C4, and equilibrium deuterium/protium fractionation factors of H3, Phi, were measured and seen to correlate with the chemical shift of the corresponding imino proton, delta(H3). Downfield-shifted imino protons associated with larger values of (2)delta(13)C2 and (2)delta(13)C4 and smaller Phi values, which together suggested that the effective H3-N3 vibrational potentials were more anharmonic in the stronger hydrogen bonds of these DNA molecules. We anticipate that (2)delta(13)C2, (2)delta(13)C4 and Phi values can be useful gauges of hydrogen bond strength of A:T base pairs.

authors

• Dunbar, Kim

published proceedings

• J Biomol NMR

altmetric score

• 0.25

author list (cited authors)

• Vakonakis, I., Salazar, M., Kang, M., Dunbar, K. R., & LiWang, A. C.

citation count

• 9

complete list of authors

• Vakonakis, Ioannis||Salazar, Miguel||Kang, Mijeong||Dunbar, Kim R||LiWang, Andy C

publication date

• February 2003

publisher

• Springer Nature  Publisher

published in

• Journal of Biomolecular NMR  Journal

keywords

• Adenine
• Base Pairing
• DNA
• Deuterium
• Genetic Code
• Hydrogen Bonding
• Isotope Labeling
• Kinetics
• Magnetic Resonance Spectroscopy
• Nucleic Acid Conformation
• Thymine

PubMed Central ID

• 12652119

Digital Object Identifier (DOI)

• 10.1023/a:1022211927051

start page

• 105

end page

• 112

volume

• 25

issue

• 2

URL

• http://dx.doi.org/10.1023/a:1022211927051