Differentiation of isotopically labeled nucleotides using fast atom bombardment tandem mass spectrometry. Academic Article uri icon

abstract

  • The positional isotope exchange reaction has proven to be a valuable tool In elucidating mechanistic pathways for enzyme-catalyzed reactions involving phosphoryl transfer. Several examples of the analysis of phosphorylated nucleotides by fast atom bombardment ionization mass spectrometry have been reported; however, the small number and low relative abundance of structurally significant fragment ions make structure elucidation difficult. Recently, we reported that the dissociation reactions of organo-alkall-metal ion complexes are influenced by the alkalt-metal binding site, and this effect can enhance the relative abundance of structurally significant fragment ions in the collision-induced dissociation spectrum. In the present studies the [M + Na]+ ions of triphosphate formed by fast atom bombardment ionization and analyzed by using tandem mass spectrometry are examined to determine the position of 18O atoms in the molecule. 1987, American Chemical Society. All rights reserved.

published proceedings

  • Anal Chem

author list (cited authors)

  • Mallis, L. M., Raushel, F. M., & Russell, D. H.

citation count

  • 15

complete list of authors

  • Mallis, LM||Raushel, FM||Russell, DH

publication date

  • April 1987