Expression of Pseudomonas phosphotriesterase activity in the fall armyworm confers resistance to insecticides. Academic Article

abstract

• The gene encoding for the phosphotriesterase (opd) from Pseudomonas diminuta has been subcloned into a baculovirus expression system. Functional enzyme is produced when the recombinant baculovirus is used to infect either cultured Spodoptera frugiperda sf9 cells or the larval stage of the fall armyworm. The LD50 for paraoxon toxicity was found to increase 280-fold in the larvae after infection with the recombinant baculovirus and expression of the functional phosphotriesterase.

authors

• Raushel, Frank

published proceedings

• Experientia

altmetric score

• 3

author list (cited authors)

• Dumas, D. P., Wild, J. R., & Raushel, F. M.

citation count

• 20

complete list of authors

• Dumas, DP||Wild, JR||Raushel, FM

publication date

• July 1990

publisher

• Springer Nature  Publisher

keywords

• Animals
• Aryldialkylphosphatase
• Genetic Vectors
• Insect Viruses
• Insecticide Resistance
• Lepidoptera
• Paraoxon
• Phosphoric Monoester Hydrolases
• Pseudomonas
• Recombinant Proteins

PubMed Central ID

• 2164956

Digital Object Identifier (DOI)

• 10.1007/BF01939948

start page

• 729

end page

• 731

volume

• 46

issue

• 7

URL

• http://dx.doi.org/10.1007/bf01939948