Development of a green fluorescent protein-based laboratory curriculum. Academic Article uri icon

abstract

  • A laboratory curriculum has been designed for an undergraduate biochemistry course that focuses on the investigation of the green fluorescent protein (GFP). The sequence of procedures extends from analysis of the DNA sequence through PCR amplification, recombinant plasmid DNA synthesis, bacterial transformation, expression, isolation, and characterization of the protein by SDS-PAGE. A survey of participants found that the majority of them were performing most of these procedures for the first time and that participants found the exercises enjoyable and considered them a significant aid to their understanding of biochemistry, cell biology, and molecular genetics.

published proceedings

  • Biochem Mol Biol Educ

author list (cited authors)

  • Larkin, P. D., & Hartberg, Y.

citation count

  • 14

complete list of authors

  • Larkin, Patrick D||Hartberg, Yasha

publication date

  • January 2005

publisher