Use of Hydrogenophaga pseudoflava penetration to quantitatively assess the impact of filtration parameters for 0.2-micrometer-pore-size filters. Academic Article uri icon


  • Filters rated as having a 0.2-microm pore size (0.2-microm-rated filters) are used in laboratory and manufacturing settings for diverse applications of bacterial and particle removal from process fluids, analytical test articles, and gasses. Using Hydrogenophaga pseudoflava, a diminutive bacterium with an unusual geometry (i.e., it is very thin), we evaluated passage through 0.2-microm-rated filters and the impact of filtration process parameters and bacterial challenge density. We show that consistent H. pseudoflava passage occurs through 0.2-microm-rated filters. This is in contrast to an absence of significant passage of nutritionally challenged bacteria that are of similar size (i.e., hydrodynamic diameter) but dissimilar geometry.

published proceedings

  • Appl Environ Microbiol

altmetric score

  • 3

author list (cited authors)

  • Lee, A., McVey, J., Faustino, P., Lute, S., Sweeney, N., Pawar, V., ... Hussong, D.

citation count

  • 7

complete list of authors

  • Lee, A||McVey, J||Faustino, P||Lute, S||Sweeney, N||Pawar, V||Khan, M||Brorson, K||Hussong, D

publication date

  • February 2010