An allylic/acyclic adenosine nucleoside triphosphate for termination of DNA synthesis by DNA template-dependent polymerases. Academic Article

abstract

• An allylic adenosine triphosphate analog (AATP) was tested as a substrate for commercially available DNA polymerases. All but one of the enzymes assayed incorporated AATP opposite thymidine (T) with concomitant termination of the elongation reaction. A concentration of only 1 microM was sufficient for complete termination of the polymerization reaction for a short template mediated by Ampli Taq DNA polymerase FS (Taq FS). This result suggests that AATP could be used as a 2',3'-dideoxyadenosine-5'-triphosphate (ddA) surrogate. Kinetics of incorporation revealed that AATP was 48 times less efficiently incorporated than ddA. Furthermore, AATP was used in dye-primer sequencing as a substitute for ddA.

authors

• Burgess, Kevin

published proceedings

• Nucleic Acids Res

altmetric score

• 3

author list (cited authors)

• Martinez, C. I., Thoresen, L. H., Gibbs, R. A., & Burgess, K.

citation count

• 7

complete list of authors

• Martinez, CI||Thoresen, LH||Gibbs, RA||Burgess, K

publication date

• March 1999

publisher

• Oxford University Press (OUP)  Publisher

published in

• Nucleic Acids Research  Journal

keywords

• Adenosine Triphosphate
• Base Sequence
• Boron Compounds
• DNA
• DNA Primers
• DNA Replication
• DNA-Directed DNA Polymerase
• Fluorescent Dyes
• Kinetics
• Substrate Specificity
• Templates, Genetic

PubMed Central ID

• 9973613

Digital Object Identifier (DOI)

• 10.1093/nar/27.5.1271

start page

• 1271

end page

• 1274

volume

• 27

issue

• 5

URL

• http://dx.doi.org/10.1093/nar/27.5.1271