Correlations of structure and rates of energy transfer for through-bond energy-transfer cassettes. Academic Article uri icon

abstract

  • Fluorescent DNA-labeling cassettes are designed to have a common absorbing chromophore matched to a single exciting laser wavelength, but up to four different emitters. Experiments reported here have examined the energy-transfer rates and fluorescence polarization characteristics for two different types of cassette, involving three distinct relative orientations of the donor and acceptor transition moments and the axis of the rigid linker. Energy-transfer times range from <200 fs to approximately 20 ps, the fastest transfer times occurring when the transition moments of the donor and acceptor species are aligned parallel to the linker axis. Experimental evidence is presented that supports a through-bond energy-transfer mechanism, in contrast with a commercial DNA-labeling agent, which exhibits much slower transfer times controlled by FRET. These rigid cassettes also exhibit polarized fluorescence from the acceptor species, so that this particular type of DNA-labeling probe has some of the advantages of single-molecule probes such as rhodamine and coumarin dyes.

published proceedings

  • J Phys Chem A

altmetric score

  • 3

author list (cited authors)

  • Kim, T. G., Castro, J. C., Loudet, A., Jiao, J., Hochstrasser, R. M., Burgess, K., & Topp, M. R.

citation count

  • 80

complete list of authors

  • Kim, TG||Castro, JC||Loudet, A||Jiao, JG-S||Hochstrasser, RM||Burgess, K||Topp, MR

publication date

  • December 2005