Intracellular imaging of organelles with new water -soluble benzophenoxazine dyes
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Five new water-soluble derivatives of Nile Red 1-5 were prepared. These benzophenoxazine dyes fluoresce between 640 and 667 nm with quantum yields of 0.17-0.33 in pH 7.4 phosphate buffer, and at slightly shorter wavelengths and higher quantum yields in EtOH. Two dyes, 3 and 4 permeated into Clone 9 cells and selectively stained mitochondria and golgi, respectively.
author list (cited authors)
Jose, J., Loudet, A., Ueno, Y., Barhoumi, R., Burghardt, R. C., & Burgess, K.