Guo, Jinbai (2003-05). Control of cell division by nutrients, and ER stress signaling in Saccharomyces cerevisiae. Doctoral Dissertation. Thesis uri icon

abstract

  • Cell cycle progression of Saccharomyces cerevisiae cells was monitored in continuous cultures limited for glucose or nitrogen. The G1 cell cycle phase, before initiation of DNA replication, did not exclusively expand when growth rate decreased. Especially during nitrogen limitation, non-G1 phases expanded almost as much as G1. In addition, cell size remained constant as a function of growth rate. These results contrast with current views that growth requirements are met before initiation of DNA replication, and suggest that distinct nutrient limitations differentially impinge on cell cycle progression. Therefore, multiple mechanisms are hypothesized to regulate the coordination of cell growth and cell division. Genetic interactions were identified between the dose-dependent cell-cycle regulator 2 (DCR2) phosphatase and genes involving in secretion/unfolded protein response pathway, including IRE1, through a genome-wide dominant negative genetic approach. Accumulation of unfolded proteins in the endoplasmic reticulum triggers the unfolded protein response (UPR). How the UPR is downregulated is not well understood. Inositol requirement 1 (IRE1) is an endoplasmic reticulum transmembrane UPR sensor in Saccharomyces cerevisiae. When the UPR is triggered, Ire1p is autophosphorylated, on Ser 840 and Ser 841, inducing the cytosolic endonuclease activity of Ire1p, thereby initiating the splicing and translational de-repression of HAC1 mRNA. Homologous to Atf/Creb1 (Hac1p) activates UPR transcription. We found that that Dcr2p phosphatase functionally and physically interacts with Ire1p. Overexpression of DCR2, but not of a catalytically inactive DCR2 allele, significantly delays HAC1 splicing and sensitizes cells to the UPR. Furthermore, Dcr2p physically interacts in vivo with Ire1p-S840E, S841E, which mimics phosphorylated Ire1p, and Dcr2p dephosphorylates Ire1p in vitro. Our results are consistent with de-phosphorylation of Ire1p being a mechanism for antagonizing UPR signaling.
  • Cell cycle progression of Saccharomyces cerevisiae cells was monitored in
    continuous cultures limited for glucose or nitrogen. The G1 cell cycle phase, before
    initiation of DNA replication, did not exclusively expand when growth rate decreased.
    Especially during nitrogen limitation, non-G1 phases expanded almost as much as G1. In
    addition, cell size remained constant as a function of growth rate. These results contrast
    with current views that growth requirements are met before initiation of DNA replication,
    and suggest that distinct nutrient limitations differentially impinge on cell cycle
    progression. Therefore, multiple mechanisms are hypothesized to regulate the
    coordination of cell growth and cell division.
    Genetic interactions were identified between the dose-dependent cell-cycle
    regulator 2 (DCR2) phosphatase and genes involving in secretion/unfolded protein
    response pathway, including IRE1, through a genome-wide dominant negative genetic
    approach. Accumulation of unfolded proteins in the endoplasmic reticulum triggers the
    unfolded protein response (UPR). How the UPR is downregulated is not well
    understood. Inositol requirement 1 (IRE1) is an endoplasmic reticulum transmembrane UPR sensor in Saccharomyces cerevisiae. When the UPR is triggered, Ire1p is
    autophosphorylated, on Ser 840 and Ser 841, inducing the cytosolic endonuclease
    activity of Ire1p, thereby initiating the splicing and translational de-repression of HAC1
    mRNA. Homologous to Atf/Creb1 (Hac1p) activates UPR transcription. We found that
    that Dcr2p phosphatase functionally and physically interacts with Ire1p. Overexpression
    of DCR2, but not of a catalytically inactive DCR2 allele, significantly delays HAC1
    splicing and sensitizes cells to the UPR. Furthermore, Dcr2p physically interacts in vivo
    with Ire1p-S840E, S841E, which mimics phosphorylated Ire1p, and Dcr2p dephosphorylates
    Ire1p in vitro. Our results are consistent with de-phosphorylation of
    Ire1p being a mechanism for antagonizing UPR signaling.

publication date

  • May 2003