Beta-galactosidase staining in the skeleton. Academic Article

abstract

• The lacZ gene, encoding for the -galactosidase enzyme, is widely used as a reporter gene in bone biology due to the ease of visualization in situ on whole-mount or on tissue sections. In this protocol we provide detailed methods for visualizing this reporter gene for both in vivo and in vitro studies. In addition, we describe a few tips for troubleshooting for mineralized tissues such as the skeleton.

authors

• Feng, Jian

published proceedings

• Methods Mol Biol

author list (cited authors)

• Han, X. L., & Feng, J. Q.

citation count

• 2

complete list of authors

• Han, XL||Feng, Jian Q

publication date

• January 2014

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Beta-Galactosidase
• Bone And Bones
• Fetus
• Histocytochemistry
• Mice
• Staining And Labeling

PubMed Central ID

• 24482173

Digital Object Identifier (DOI)

• 10.1007/978-1-62703-989-5_13

start page

• 185

end page

• 191

volume

• 1130

URL

• http://dx.doi.org/10.1007/978-1-62703-989-5_13