Wnt3 and transforming growth factor- induce myofibroblast differentiation from periodontal ligament cells via different pathways. Academic Article uri icon


  • Myofibroblasts are specialized cells that play a key role in connective tissue remodeling and reconstruction. Alpha-smooth muscle actin (-SMA), vimentin and tenascin-C are myofibroblast phenotype, while -SMA is the phenotypic marker. The observation that human periodontal ligament cells (hPDLCs) differentiate into myofibroblasts under orthodontic force has provided a new perspective for understanding of the biological and biomechanical mechanisms involved in orthodontic tooth movement. However, the cell-specific molecular mechanisms leading to myofibroblast differentiation in the periodontal ligament (PDL) remain unclear. In this study, we found that expression of Wnt3, transforming growth factor-1 (TGF-1), -SMA and tenascin-C increased in both tension and compression regions of the PDL under orthodontic load compared with unloaded control, suggesting that upregulated Wnt3 and TGF-1 signaling might have roles in myofibroblast differentiation in response to orthodontic force. We reveal in vitro that both Wnt3 and TGF-1 promote myofibroblast differentiation from hPDLCs. Dickkopf-1 (DKK1) impairs Wnt3-induced myofibroblast differentiation in a -catenin-dependent manner. TGF-1 stimulates myofibroblast differentiation via a JNK-dependent mechanism. DKK1 has no significant effect on TGF-1-induced myofibroblastic phenotype.

published proceedings

  • Exp Cell Res

author list (cited authors)

  • Xu, H., He, Y., Feng, J. Q., Shu, R., Liu, Z., Li, J., ... Han, X.

citation count

  • 10

complete list of authors

  • Xu, Hui||He, Yao||Feng, Jian Q||Shu, Rui||Liu, Zhe||Li, Jingyu||Wang, Yating||Xu, Yang||Zeng, Huan||Xu, Xin||Xiang, Zichao||Xue, Chaoran||Bai, Ding||Han, Xianglong

publication date

  • April 2017