Optimization of fermentation parameters using response surface methodology for biohydrogen production from urban waste Academic Article uri icon


  • 2018 Politechnica University of Bucharest. All rights reserved. Biohydrogen was prepared by batch fermentation and the parameters for anaerobic fermentation under mesophilic condition were optimized using Design Expert Software version 7.0.0. Central composite design of response surface methodology was used to optimize parameters like weight of substrate, weight of culture and time. The substrate used was food waste and the culture were a mixed culture obtained from cow dung and industrial effluent. Biohydrogen was obtained at optimized parameters of 96 hours with 125 g of food waste and 125 g of cow dung. The coefficient of regression 0.9979 and the adjusted R 2 value of 0.9956 is in good agreement with predicted R 2 value of 0.9957. The investigation was done by dark fermentation and the composition of gas produced was determined using gas chromatography which confirmed the presence of biohydrogen of 26.87% yield.

published proceedings

  • UPB Scientific Bulletin, Series B: Chemistry and Materials Science

author list (cited authors)

  • Annam Renita, A., Kumar, N., & Salla, S.

complete list of authors

  • Annam Renita, A||Kumar, N||Salla, S

publication date

  • January 2018