Simple and sensitive high-performance liquid chromatographic method for determination of transdermally applied flurbiprofen in rat plasma and excised skin samples Academic Article uri icon

abstract

  • A simple reversed-phase HPLC method has been developed for determination of flurbiprofen in rat plasma, excised skin extract, and transdermal patch formulations. The mobile phase was methanol-1% (v/v) phosphoric acid in water, 80:20 (v/v), at a flow rate of 0.5 mL min-1; ibuprofen was used as internal standard. Flurbiprofen and ibuprofen was detected by UV absorption at 254 nm and 220 nm, respectively. The limit of quantitation was 0.1 g mL -1. The response was linearly dependent on concentration in the range 0.1-10 g mL-1, and accuracy and reproducibility were good. At these concentrations intraday and interday assay variability were below 8%. Recovery of flurbiprofen was greater than 94% over the linear range of calibration plot. 2005 Friedr. Vieweg & Sohn/GWV Fachverlage GmbH.

published proceedings

  • CHROMATOGRAPHIA

author list (cited authors)

  • Charoo, N. A., Shamsher, A., Kohli, K., Pillai, K. K., & Rahman, Z.

citation count

  • 8

complete list of authors

  • Charoo, NA||Shamsher, AAA||Kohli, K||Pillai, KK||Rahman, Z

publication date

  • November 2005