Studies on 16-Hydroxylation of Steroid Molecules and Regioselective Binding Mode in Homology-Modeled Cytochrome P450-2C11.
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We investigated the 16-hydroxylation of steroid molecules and regioselective binding mode in homology-modeled cytochrome P450-2C11 to correlate the biological study with the computational molecular modeling. It revealed that there was a positive relationship between the observed inhibitory potencies and the binding free energies. Docking of steroid molecules into this homology-modeled CYP2C11 indicated that 16-hydroxylation is favored with steroidal molecules possessing the following components, (1) a bent A-B ring configuration (5-reduced), (2) C-3 -hydroxyl group, (3) C-17-acetyl group, and (4) methyl group at both the C-18 and C-19. These respective steroid components requirements were defined as the inhibitory contribution factor. Overall studies of the male rat CYP2C11 metabolism revealed that the above-mentioned steroid components requirements were essential to induce an effective inhibition of [(3)H]progesterone 16-hydroxylation. As far as docking of homology-modeled CYP2C11 against investigated steroids is concerned, they are docked at the active site superimposed with flurbiprofen. It was also found that the distance between heme iron and C16-H was between 4 to 6 and that the related angle was in the range of 180 45.
author list (cited authors)
Ali, H. I., Yamada, M., Fujita, Y., Maeda, M., & Akaho, E.
complete list of authors
Ali, Hamed I||Yamada, Morio||Fujita, Yukihisa||Maeda, Mitsuko||Akaho, Eiichi