- COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only. In this paper, we report a new photoacoustic sensing probe design consisting of two optical fibers. One optical fiber is used for delivering the excitation light pulses. The other one serves as an acoustic delay line to relay the generated PA signal from the target to an outside ultrasound transducer. With the addition of suitable time delay, the original PA signal can be easily separated from the interference signals. To demonstrate this new design, a prototype probe was designed, fabricated and tested. The PA sensing performance was characterized with different concentration of black dye solutions. The testing results show that the PA sensing probe can provide good sensitivity and can maintain high linearity over a wide range of concentrations.