Optimization of batch fermentor sterilization. Academic Article uri icon


  • This article presents a calculation procedure useful for the optimization and scale up of batch sterilization cycles in large-scale fermentors. This technique determines the sterilization temperature and hold-time necessary to minimize nutrient damage in a specific fermentor. The method can also be used for "scaledown" experiments to eliminate sterilization conditions as a scale up parameter. A method for the systematic evaluation of different sterilization conditions on product yield is also presented. This procedure is useful in determining if scale up of sterilization conditions is important for a given process. The validity of the techniques presented are supported by data showing significant yield improvements in a 1.2 x 10(5) L antibiotic fermentation.

published proceedings

  • Biotechnol Bioeng

author list (cited authors)

  • Singh, V., Hensler, W., & Fuchs, R.

citation count

  • 8

complete list of authors

  • Singh, V||Hensler, W||Fuchs, R

publication date

  • January 1989