High-resolution melting analysis (HRMA): a highly sensitive inexpensive genotyping alternative for population studies. uri icon

abstract

  • High-resolution melting analysis (HRMA) is a highly sensitive closed-tube genotyping method used primarily in clinical studies. As the method is rapid, inexpensive and amenable to high throughput, we decided to investigate its applicability to population studies. Small amplicons and unlabelled probes were used to genotype the nuclear genes, lactate dehydrogenase-A (ldh-A), myosin light chain-2 (mlc-2), acidic ribosomal phosphoprotein P0 (ARP) and calmodulin (CaM) in populations of swordfish, Xiphias gladius. Results indicate that HRMA is a powerful genotyping tool to study wild populations.

published proceedings

  • Mol Ecol Resour

altmetric score

  • 3

author list (cited authors)

  • Smith, B. L., Lu, C., & Alvarado Bremer, J. R.

citation count

  • 54

complete list of authors

  • Smith, BL||Lu, C-P||Alvarado Bremer, JR

publication date

  • January 2010

publisher