Shake and stew: a non-destructive PCR-ready DNA isolation method from a single preserved fish larva. Academic Article uri icon

abstract

  • A rapid non-destructive alternative to isolate DNA from an individual fish larva is presented, based on the suspension of epithelial cells through vortex forces, and the release of DNA in a heated alkaline solution. DNA from >6056 fish larvae isolated using this protocol has yielded a high PCR amplification success rate (>93%), suggesting its applicability to other taxonomic groups or sources when tissue amount is the limiting factor.

published proceedings

  • J Fish Biol

altmetric score

  • 0.5

author list (cited authors)

  • Alvarado Bremer, J. R., Smith, B. L., Moulton, D. L., Lu, C., & Cornic, M.

citation count

  • 7

complete list of authors

  • Alvarado Bremer, JR||Smith, BL||Moulton, DL||Lu, C-P||Cornic, M

publication date

  • January 2014

publisher