Genotyping live fish larvae: Non-lethal and noninvasive DNA isolation from 3-5 day old hatchlings. Academic Article uri icon

abstract

  • Genotyping fish larvae is a valuable technique for numerous fields of study. While methods for collecting DNA from early stage larvae have been published, a non-lethal, non-invasive genotyping protocol for hatchlings that is amenable to high-throughput approaches is desirable. Here, we describe a method to individually genotype live, free-swimming, early fish larvae by characterizing their environmental DNA (eDNA). We demonstrate the utility of the method by assigning parentage to a sample (n = 50) of 3-5-day-old sheepshead minnow (Cyprinodon variegatus) larvae hatchlings, with very high rates of genotyping success (98%) and survival (92%) using mitochondrial and microsatellite DNA data. This method could be easily adapted to characterize early fish larvae from other model and non-model fish species, such as Danio rerio (zebrafish) and Medaka medaka.

published proceedings

  • Biotechniques

altmetric score

  • 1.5

author list (cited authors)

  • Espinoza, G. J., Poland, J. M., & Bremer, J.

citation count

  • 4

complete list of authors

  • Espinoza, G Janelle||Poland, J Michael||Bremer, Jaime R Alvarado

publication date

  • January 1, 2017 11:11 AM