JADAS: a customizable automated data acquisition system and its application to ice-embedded single particles. Academic Article uri icon

abstract

  • The JEOL Automated Data Acquisition System (JADAS) is a software system built for the latest generation of the JEOL Transmission Electron Microscopes. It is designed to partially or fully automate image acquisition for ice-embedded single particles under low dose conditions. Its built-in flexibility permits users to customize the order of various imaging operations. In this paper, we describe how JADAS is used to accurately locate and image suitable specimen areas on a grid of ice-embedded particles. We also demonstrate the utility of JADAS by imaging the epsilon 15 bacteriophage with the JEM3200FSC electron cryo-microscope, showing that sufficient images can be collected in a single 8h session to yield a subnanometer resolution structure which agrees with the previously determined structure.

published proceedings

  • J Struct Biol

author list (cited authors)

  • Zhang, J., Nakamura, N., Shimizu, Y., Liang, N., Liu, X., Jakana, J., ... Chiu, W.

citation count

  • 54

complete list of authors

  • Zhang, Junjie||Nakamura, Natsuko||Shimizu, Yuko||Liang, Nathan||Liu, Xiangan||Jakana, Joanita||Marsh, Michael P||Booth, Christopher R||Shinkawa, Takao||Nakata, Munetaka||Chiu, Wah

publication date

  • January 2009